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21:15 Oct 20, 2017 |
Russian to English translations [PRO] Medical - Medical (general) / Clinical Trial on Pharmacokinetics | |||||||
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| Selected response from: Natalie Poland Local time: 12:03 | ||||||
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Summary of answers provided | ||||
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4 +1 | system priming |
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3 | Filling the system |
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Filling the system Explanation: |
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system priming Explanation: * -------------------------------------------------- Note added at 18 hrs (2017-10-21 16:07:17 GMT) -------------------------------------------------- "Priming" means initial filling of an empty system with whatever is needed: solution, buffer, blood, oil, etc., depending on what is used in that system. Examples for chromatography system: Analytical Techniques in Immunochemistry - Google Books Result https://books.google.ca/books?isbn=0824784774 Terry M. Phillips - 1991 - Science Fast Protein Liquid Chromatography 1. Fill the ... Prime the system by filling the tubing from buffer reservoir I with a syringe, connected to the pump inlet valve. biologic duoflow™ chromatography system - Bio-Rad www.bio-rad.com/webroot/web/pdf/lsr/literature/4006229.pdf The BioLogic DuoFlow chromatography system is specifically designed for the ...... used to prime the system, calibrate the pH and conductivity monitor, observe ... NGC Chromatography Systems and ChromLab Software ... - Bio-Rad www.bio-rad.com/webroot/web/pdf/lsr/literature/10026253.pdf Guide is available on your NGC Chromatography Systems Documentation ...... Caution: Prime the system before connecting a column to the NGC instrument. Why Agilent HPLC pump not building pressure?? - ResearchGate https://www.researchgate.net/.../Why_Agilent_HPLC_pump_not_b... pressure First of all, make sure you have sufficient solvent in the solvent bottle. Prime the system by running with the purge valve open for several minutes. Good chromatographic practices - SlideShare https://www.slideshare.net/skvemula/good-chromatographic-pra... 16 Dec 2013 ... Good chromatographic practices to be followed mainly for HPLC. ... with Milli-Q water and wet prime the system for 10 minutes at 3 mL/min. -------------------------------------------------- Note added at 18 hrs (2017-10-21 16:08:33 GMT) -------------------------------------------------- Primers (used in molecular biology) have nothing to do with this :-) -------------------------------------------------- Note added at 19 hrs (2017-10-21 16:18:14 GMT) -------------------------------------------------- Priming of the system is used to expel air from various tubings etc. See HPLC manual, for example: Agilent 1100 Series HPLC Value System User's Guide http://tinyurl.com/ya7d9rtg Priming and Purging the System When the solvents have been exchanged or the pumping system has been turned off for a certain time (for example, overnight) oxygen will re-diffuse into the solvent channel between the solvent reservoir, vacuum degasser (when available in the system) and the pump. Solvents containing volatile ingredients will slightly lose these. Therefore priming of the pumping system is required before starting an application. The pump should never be used for priming empty tubings (never let the pump run dry). Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump. -------------------------------------------------- Note added at 19 hrs (2017-10-21 16:39:05 GMT) -------------------------------------------------- The principle is exactly the same: you need to take somewhat more blood and to prime the tubings before analysis. If the analyzed blood sample is, for example, 1 ml, and the volume of the tubings to be primed is also 1 ml, you need 2 ml of blood total -------------------------------------------------- Note added at 19 hrs (2017-10-21 16:49:55 GMT) -------------------------------------------------- Here is a manual for a HPLC-based whole blood glycohemoglobin analyzer: http://tinyurl.com/ya4sqav2 page 13: 13. Before calibrating the newly installed column, analyze at least three whole blood samples to prime the column. Calibrate the system and 5 duplicate specimens and controls. page 14: After column replacement Analyze three whole blood specimens to prime the column. page 16-17: Let syringes cycle 4-5 times to purge tubing and syringes of air and to prime the system with reagent. page 19: The analyzer will prime the fluid lines with buffer, then analyze samples at 1.6 minute intervals. The analyzer aspirates 4 μL of whole blood. The whole blood sample is automatically diluted approximately 1:200 and introduced into the assay line. page 20: If a new lot number of column is installed, analyze three whole blood specimens to prime the column. |
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